Only if they feel threatened. For good measure we usually smoke them, this masks any alarm pheromone from the guards and causes the bees to think there is a forest fire and load up their bellies with honey, making them sluggish and drowsy.
Then as long as you don’t make quick sudden movements or murder too many of them they will let you handle them. When I learned beekeeping I used the gloves once before I ditched them. They are clumsy and I tend to accidentally squish the poor girls with them.
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