Only if they feel threatened. For good measure we usually smoke them, this masks any alarm pheromone from the guards and causes the bees to think there is a forest fire and load up their bellies with honey, making them sluggish and drowsy.
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Then as long as you don’t make quick sudden movements or murder too many of them they will let you handle them. When I learned beekeeping I used the gloves once before I ditched them. They are clumsy and I tend to accidentally squish the poor girls with them.
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